Plant Growth Regulators – Plant Tissue Culture Protocol

The importance of plant growth regulators in plant tissue culture is well documented.  CAISSON Laboratories, Inc. (CAISSON Labs) offers a broad range of plant growth regulators specifically tested for plant cell culture.  Each product is biologically tested following the criteria as established for powdered media.  Each cytokinin is tested for stimulation of shoot production.

The plant growth regulator solutions are sterile filtered through a 0.2 µm filter unit into sterile bottles.  Each lot of plant regulator solution is tested for sterility according to specifications established for U.S. Pharmacopeia, as well as biological testing in plant cell culture using criteria identical to those established for our plant tissue culture media.

Disclaimer: These products are for laboratory, plant tissue culture media preparation, and plant research purposes only.  Not for use as a plant growth regulator on developed plants.  They are not for drug or household use.

Preparation and use:

To prepare a 1 mg/ml stock solution: Add 100 mg of the plant growth regulator to a 100 ml flask or another glass container.  Add 2-5 ml of solvent to dissolve the powder.  Once completely dissolved, bring to volume with cell culture grade water like our catalog# WAL01.  Stirring the solution while adding water may be required to keep the material in the solution.  Store the stock solution as recommended in the tables.  Add 1.0 ml of the stock solution to 1 liter of medium to obtain a final concentration of 1.0 mg/l of the plant growth regulator in the culture medium.  (See table below.)

Volume of stock solution = (Desired Hormone Concentration X Medium Volume) / Stock Solution Concentration

Plant cell culture Tested Auxins are generally used in plant cell culture at a concentration range of 0.1 – 10.0 mg/L.  When added in appropriate concentrations, they may regulate cell elongation, tissue swelling, cell division, formation of adventitious roots, and inhibition of adventitious and axillary shoot formation, callus initiation and growth, and induction of embryogenesis.

Plant cell culture tested Cytokinins are generally used in plant cell culture at a concentration range of 0.1 – 10.0 mg/L.  When added in appropriate concentrations, they may regulate cell division, stimulate axillary and adventitious shoot proliferation, regulate differentiation, inhibit root formation, activate RNA synthesis and stimulate protein and enzyme activity.