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Dulbecco’s Modified Eagle’s Medium / Ham’s Nutrient Mixture F12

1. Introduction:

A basal-defined medium that properly promotes sustainable development and phenomenal growth in numerous mammalian cells and is used widely in the specific cell culturing processes [1].

  • DMEM/F-12 is typically composed of four times more necessary vitamins and amino acids as compared to the original DMEM.
  • Considerable variety of specialized cells indicate phenomenal growth and successful development in DMEM/F-12 like glial cells, neurons, fibroblast cells, smooth muscle cells, and endothelial cells of humans [2].
  • DMEM/F-12 is available in different varieties typically based on the possible presence or notable absence of certain substances shown in the table [3].
    • Without HEPES• Without Phenol Red• Stable Glutamine
    • With HEPES• With Phenol Red• Without Glutamine
    • With L-Glutamine
  • It is obtainable in various standard sizes of poly bottles packaging of 500 mL, 1 Liter, 5 Liters, 10 Liters, and 50 Liters. It is equally attainable in bulk quantities on economic demand [4].

Dulbecco’s Modified Eagle’s Medium (DMEM)