Indole-3-butyric Acid (IBA) Solution

Indole-3-Butyric Acid (IBA) Solution C₁₂H₁₃NO₂

A plant growth hormone naturally belongs to the auxins family, present in peas, maize and tissues of carrots. Indole-3-butyric acid (IBA) undoubtedly has many commercial and agricultural benefits [3].

Introduction

• Indole-3-butyric acid (IBA) Solution is scientifically derived from butyric acid. It acts appropriately as an auxin precursor, plant rooting agent, responsible growth hormone, and plant metabolite.
• Indole-3-butyric acid is obtainable in the established market under the recognizable names of various descriptive labels like 4-(1H-Indol-3-yl) butanoic acid, Indolebutyric acid, Jiffy grow, Rhizopon AA, and Hormex rooting powder [7].
• Indole-3-butyric acid (IBA) Solution is available in different sizes of poly bottles packaging of 5 grams, 10 grams, 25 grams, and 50 grams. It is equally attainable in bulk quantities [6].

Structure

The unique structure of Indole-3-butyric acid is displayed underneath [1].

Figure 1: Structure of Indole-3-butyric acid [1].

Procedure

Indole-3-butyric acid naturally occurs in the peelings of cultivated potatoes, cypress, maize, and tobacco plant. It is assuredly an endogenous component in various plant tissues. Indole-3-butyric acid (IBA) Solution naturally comes from the Arabidopsis (tiny flowering plants) [4]. The standard procedure for the possible preparation of indole-3-butyric acid is described below in considerable detail.

Apparatus and chemicals:

The apparatus and chemicals used in the preparation of stock solution of indole-3-butyric acid are given below.

Preparation method:

The necessary steps involved in the stock preparation of indole-3-butyric acid are described below [5].

1. Carefully measure 1 gram of indole-3-butyric acid in the beaker (250ml) by properly using a balance machine.
2. Instantly add 1 gram of indole-3-butyric acid (IBA) powder to the beaker. Place it on the tripod stand and agitate it gently with the help of the stirrer.
3. By properly using a volumetric flask, measure 2 to 5ml of ethyl alcohol and instantly add it to the beaker to dissolve the odorless powder completely.
4. You can additionally use sodium hydroxide to dissolve powdery IBA instead of ethyl alcohol.
5. Stir gently the solution thoroughly so that the powder can be promptly disappeared from the beaker.
6. You can properly sterilize the satisfactory solution by typically using autoclaves or by filtering it with the help of filter paper.
7. The last step in common is to properly store the stock solution at -20 degrees Celsius for future use.

Applications

The practical applications of indole-3-butyric acid are described below [2][3].

1. Plant Growth Regulator: Indole-3-butyric acid acts as a principal regulator in complex mechanisms and processes which lead to plant growth.
2. Root Formation: IBA performs a significant role in the sustainable development and successful formation of roots and root nodes in certain plants.
3. Crop Yield: IBA additionally enhances the efficient production of cultivated crops which leads to an increase in the total yield of crops.
4. Development of fruit and flowers: Standard Indole-3-butyric acid equally plays a significant role in properly stimulating the sustainable growth and proper development of blooming flowers or luscious fruits in growing plants.
5. Biochemical Pesticides: IBA is also classified as an effective pesticide because it is similar to indole-3-acetic acid, a naturally occurring growth hormone in mature plants.
6. Formation of Callus: Indole-3-butyric acid also results in the successful production of the callus (a specific group of undifferentiated cells). This callus formation then results in the complex formation of necessary tissues for generating plants like roots [3].

Specifications

The specifications of indole-3-butyric acid are described below in the table [6][8].

Hazards and Safety
The potential hazards and safety measures are described below [9] [10].

Eye Contact:
It can typically cause serious eye damage, considerable irritation, and possible infection. It can typically cause temporary injury to the eyes.

First Aid Measure:
In the specific cases of direct contact with eyes, immediately wash the eyes with plenty of distilled water. Hold the eyelids open and cleanse the face of the victim repeatedly for 15-20 minutes. In case of severe irritation or redness, adequately consult medical advice as soon as possible.

Skin Contact:
Graciously according to hazard statements, it can also cause skin corrosion, evident irritation, and mild rashes.

First Aid Measure:
In case of physical contact of the odorless powder with the intact skin of specific body parts, immediately wash the skin or specific body parts with excess water. Carefully remove the contaminated clothes immediately and properly wash them. The washed clothes can be reused. You can employ neutral soaps to progressively reduce the irritation caused by IBA. In case of severe infection, properly consult medical advice.

Respiratory Tract Infections:
According to the hazard statement, if accidentally inspired it can cause difficulty in breathing and irritation in respiratory membranes. Do not breathe dust, mist, or noxious vapors of indole-3-butyric acid.

First Aid Measure:
In case of inhalation of IBA powder, offer water to the identified victim and typically seek medical advice.

Ingestion:
According to the hazard statement, if mistakenly ingested it can cause nausea, vomiting, and a headache.

First Aid Measure:
If it is consumed mistakenly, immediately arrange some milk for the victim. If not available, then you can equally give him egg white or gelatin solution. If these things are unavailable, then give plenty of water to the victim for drinking. In such situations avoid drinking alcoholic beverages or alcohol.

Ventilated Places:
It is necessary to use well-ventilated places for the experimentation procedures.

Protection:
Use safety goggles, sterile gloves, and protective clothes like a lab coat. Properly use practical things that efficiently are chemical resistant for possible protection.

References

1. ChEBI – Indole-3-Butyric Acid
2. EPA NSCEP – R.E.D. Facts Indole-3-Butyric Acid
3. CliniSciences – Plant Growth Regulators – Auxins – Indole-3-butyric acid (IBA)
4. USDA Organic Production – Indole-3-butyric acid (IBA)
5. GoldBio – Indole-3-butyric acid (IBA)
6. Bertin BioReagent – Indole-3-butyric Acid
7. PubChem NCBI NIH – Indole-3-butyric Acid 2.4.2 Depositor-Supplied Synonyms
8. PubChem NCBI NIH – Indole-3-butyric Acid 3 Chemical & Physical Properties
9. Carl Roth – Indole-3-butyric Acid Safety Data Sheet
10. EPA NSCEP – R.E.D. Facts Indole-3-Butyric Acid