IMDM Media Formulation

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IMDM Media Formulation

A highly enriched medium that is formed by the modification in the Dulbecco’s medium [1].

1. Introduction:

  • Iscove’s Modified Dulbecco’s Medium was first prepared by N.N. Iscove in 1978. He cultured B lymphocytes (stem cells developed from bone marrow) of mice that developed to its maturation stage without even adding serums.
  • In IMDM media formation, Dulbecco’s Modified Eagle Medium quality was enhanced by adding selenium and in place of serum, it contains albumin, soya bean lipids, and transferrin.
  • Due to the presence of replacement supplements and selenium, it becomes highly enriched in amino acids, vitamins, and glucose [1].
  • It is obtainable in the market in different sizes like in the packaging of 250ml, 500ml, 1,000 ml, 10 x 500 ml, and 1 Liter. It is typically packed in sustainable plastic bottles tightly sealed [2].

Iscove’s Modified Dulbecco’s Medium (IMDM)