6-Benzylaminopurine (BAP Hormone) efficiently is a synthetic Cytokinin that in common is an adenine-based plant hormone that helps in the sustainable development of auxins. The effective concentration of 6-benzylaminopurine (BAP Hormone) used in the plant cell culture is respectively 1mg to 10mg per liter .
6-Benzylaminopurine naturally has the considerable ability to be used as a plant supplement in the plant culture media .
It is obtainable under the names of various labels like BAP (growth stimulant), Benzyl-amino purine, 6-BAP, Adenine, N-benzyl-, Cytokinin B, and N-Benzyl-9H-purin-6-amine .
It is attainable in the economic market in the packaging of 100mg, 200mg, 250mg, 500mg, 1g, 5g, and 25g in poly bottles. It is also obtainable in bulk quantities like 1kg, 5kg, and 25kg .
The structure of 6-Benzylaminopurine (BAP Hormone) is shown below.
Figure 1: Structure of 6-Benzylaminopurine .
The adequate preparation of 6-Benzylaminopurine (BAP Hormone) in the laboratory is typically done in the following way described below .
Apparatus and chemicals:
The apparatus and chemicals used in the preparation includes:
Bunsen Burner, Tripod, and Gauze
Beaker, Sterile Bottle, and Volumetric Flask
Stirrer, Evaporating Basin, Watch Glass and Filter Paper
The preparation method appropriately includes the following steps:
Carefully take a beaker and add water to it. Place the beaker on a tripod stand set on the Bunsen burner. Heat sufficiently the distilled water gently.
Typically add 2.25 grams of benzoic anhydride to the beaker with mildly heated water. Stir with help of the stirrer. Subsequently, add 1 gram of adenine to the beaker. Again stir the solution and gently heat the homogenous solution for 10 minutes.
In order to solidify the sustainable solution, typically continue to heat it for further 30 minutes. Then add water to the solution and heat it again for 25 to 30 minutes.
Instantly add sodium bicarbonate to the solution for neutralization and chill.
You can clearly note that crystallization is occurring in the beaker. The next step correctly is to filter the organic crystals.
The evaporating basin is used to evaporate the water in the solution, producing the crystals on the filter paper. By consuming another filter paper, dry the crystals at 100 degrees centigrade.
If the organic crystal melts, it can be recrystallized by using dilute alcohol or distilled water.
Stock solution preparation:
The stock solution of 6-Benzylaminopurine (BAP Hormone) is typically manufactured in the laboratory by carefully following certain necessary steps that are described below .
Typically take powder form of 6-Benzylaminopurine (100 grams) in the volumetric flask in order to adequately prepare a stock solution of 6-Benzylaminopurine (BAP Hormone) Solution.
Subsequently, carefully put 2ml to 5ml of sodium hydroxide solution in the flask to dissolve the odorless powder.
Add water to the solution and agitate it well. Keep stirring the solution until the powder dissolves completely.
The stock solution is sufficiently prepared that can be efficiently stored at temperature ranges from 2 to 8 degrees Celsius.
The practical applications of 6-Benzylaminopurine (BAP Hormone) that undoubtedly play an important role in diverse fields at the commercial level are described below .
Induce sprouting: 6-Benzylaminopurine (BAP Hormone) helps in inducing the natural process of sprouting in generating plants or plant-related material.
Seed germination: 6-Benzylaminopurine (BAP Hormone) naturally contains key constituents that properly promote the germination of fertile seeds.
Promote shoot initiation: 6-Benzylaminopurine (BAP Hormone) also plays a significant role in the initiation of growth processes of plant shoots.
Stimulate cell division: 6-Benzylaminopurine (BAP Hormone) naturally stimulates cell division in the generating plants which leads to blossoming and fruit richness in plants .
Callus culture: 6-Benzylaminopurine (BAP Hormone) undoubtedly helps in the active growth of the plant’s callus (group of specialized cells) in the culture .
Inhibits kinase in plant: 6-Benzylaminopurine (BAP Hormone) inhibits a respiratory enzyme kinase involved in the metabolic activities of a plant’s mature leaves .
The specifications of 6-Benzylaminopurine (BAP) are described in the table below .
Powdery or crystal form
White or light yellow
Soluble in water, sodium hydroxide, and dimethyl formamide
The safety measures and hazards of 6-Benzylaminopurine are described below .
Eye irritation: According to H315 and H319, 6-Benzylaminopurine can cause serious eyes irritation, itching, and redness. First Aid Measure: If the powdery form of 6-Benzylaminopurine comes in contact with eyes in the form of dust. Immediately wash your eyes water for 10 to 15 minutes. Remove contact lenses if wearing them. In case of a serious problem, consult the doctor immediately. Rinse your face properly.
Skin irritation: According to the hazard statement, it can cause itching and infection after prolonged exposure. First Aid Measure: If the skin gets contaminated from 6-Benzylaminopurine while dealing with it. Instantly wash the contaminated part with plenty of water using neutral soap along with it. Remove the contaminated clothes carefully. Rinse water thoroughly. If it causes serious problems, take the victim to a nearby hospital.
Respiratory tract irritation: According to hazard statement H335, exposure to 6-Benzylaminopurine leads to the serious issue of irritation in the respiratory tract. It is important to avoid breathing fumes, dust, spray, and powder. First Aid Measure: If the person is not breathing, take him hospital immediately. Give him artificial breathing. If he is stable and conscious move him to fresh air after exposure.
Digestive tract irritation: If it is ingested mistakenly, 6-Benzylaminopurine can cause digestive tract infections. First Aid Measure: wash the mouth of the person immediately. Give him plenty of water to drink. Take him to the hospital, so that doctors can treat him according to symptoms.
Effect aquatic life: 6-Benzylaminopurine can also affect marine and aquatic life. Dispose of the remaining contents carefully. So that it cannot affect the natural ecosystem of living organisms.