Description
MEL06 Minimum Essential Medium with Earle’s Salts (MEM) is used in animal cell culture to support the growth and maintenance of mammalian cells. Key points include:
1. **Basic Nutrients**: Provides essential nutrients like amino acids, vitamins, salts, and glucose.
2. **Earle’s Salts**: Maintains osmotic balance and supplies essential ions, helping to maintain physiological pH with sodium bicarbonate as a buffering agent.
3. **Versatility**: Suitable for a wide range of cell types, including primary and established cell lines, both adherent and suspension cells.
4. **Supplementation**: Often supplemented with fetal bovine serum (FBS), growth factors, or other specific components depending on cell requirements.
5. **Applications**: Used for cell maintenance, proliferation studies, cytotoxicity assays, and metabolic studies.
In summary, MEM with Earle’s Salts provides the essential nutrients and conditions needed for the optimal growth and function of animal cells in culture.
