Description
HBP01 Hank’s Balanced Salts (HBS) is a commonly used buffer in cell culture and tissue handling. It provides cells with essential inorganic ions and a balanced pH environment, which are critical for maintaining cellular functions and viability outside of their natural environment. Here are some specific uses of HBSS in cell culture:
1. **Cell Washing**: HBS is often made into a solution used to wash cells to remove serum, dead cells, and other unwanted components before further processing, such as passaging or experimental treatments.
2. **Transportation and Storage**: It can be used to transport and temporarily store cells and tissues. The balanced salts help to maintain osmotic balance and provide essential ions during these processes.
3. **Dissociation of Cells**: HBS is employed in combination with enzymes like trypsin or collagenase to dissociate cells from tissue or culture surfaces.
4. **Preparation of Reagents**: Many cell culture reagents and solutions are prepared using HBS as a base due to their balanced ion composition and physiological pH.
5. **Experimental Assays**: It is used in various experimental assays that require a controlled environment, such as measuring cellular responses to stimuli or performing biochemical assays.
6. **In Vivo Studies**: In some cases, HBS is used in in vivo studies for perfusion or as a vehicle for drug delivery because it mimics the osmolarity and ion concentration of physiological fluids.
This particular formulation contains Calcium, Magnesium, and Phenol Red. Please consult with experts when using this product with specialized applications. Results may vary depending on the specifications of the experiment.
