With Earle’s salts and L-glutamine. No phenol red.

With Earle’s salts. Without L-glutamine.

With Earle’s Salts, L-Glutamine, Non_Essential Amino Acids, and 1500mg/L Sodium Bicarbonate. Without L-Arginine, and L-Lysine.

Without L-Arginine, L-Glutamine, L-Lysine and Phenol Red.

With 20mM HEPES, 1000 U/L Penicillin G, 1mg/L Streptomycin and 1mM L-Alanyl-L-Glutamine

Without L-glutamine and phenol red.

With high glucose, L-glutamine, and 10mM HEPES, sodium pyruvate and 1500mg/L sodium bicarbonate.