Description
T019 Trypsin 1:250 is used in the preparation of trypsin-EDTA solution, which is commonly utilized for detaching adherent cells from the culture vessel for subculturing or passaging. Please see some applications below:
1. **Cell detachment**: T019 Trypsin 1:250 is an enzyme that breaks down proteins, including the proteins that anchor cells to the culture vessel. When cells reach confluence or need to be passaged, trypsin-EDTA solution is added to the cell culture to detach the cells from the surface, allowing them to be collected and transferred to a new culture vessel for further growth.
2. **Passaging**: During cell passaging, the trypsin-EDTA treatment helps to dissociate cells from each other and the culture dish, allowing them to be separated into single cells or small clusters. This process is essential for maintaining healthy and viable cell cultures over successive generations.
3. **Cell harvesting**: Trypsin-EDTA treatment is also used for harvesting cells for downstream applications such as cell counting, analysis, or experimentation.
By using a 1:250 dilution of trypsin, researchers can achieve an optimal concentration of trypsin-EDTA for efficient cell detachment while minimizing the potential for over-digestion or damage to the cells during the detachment process.
**Disclaimer:** Results may vary depending on specific cell lines and experimental conditions. Consult with cell culture specialists and review comprehensive literature to tailor T019’s use to your specific research needs.
