RPMI 1640 Medium (Modification 12)

RPP12 RPMI 1640 Medium (Modification 12) contains L-glutamine and 25 mM HEPES, but does not contain sodium bicarbonate–and is a versatile and essential medium widely utilized in animal cell culture, particularly for the growth and maintenance of various cell types. This enriched formulation is designed to provide an optimal environment that supports cellular metabolism, proliferation, and function. The addition of L-glutamine serves as a vital amino acid that promotes cell growth, while HEPES buffers maintain stable pH levels, ensuring a conducive environment for sensitive cells. This medium is ideal for applications such as immunology, cancer research, and bioproduction, enabling researchers to explore cellular responses, study disease mechanisms, and produce therapeutic proteins.