Description
MBP02 MCDB 131 Medium (Modification 2) does not contain Glucose, L-Glutamine, Phenol Red, and Sodium Bicarbonate. It is a defined, serum-free culture medium widely used for the growth and maintenance of various mammalian cell types, including endothelial cells and certain cancer cell lines. When this medium is modified to exclude glucose, L-glutamine, phenol red, and sodium bicarbonate, it serves distinct purposes in cell culture, tailored to specific cell types and applications:
1. Absence of Glucose
- Metabolic Studies with Hepatocytes: Hepatocytes (liver cells) are often used to study metabolic pathways related to glucose deprivation, such as gluconeogenesis.
- Cancer Research with Breast Cancer Cells (MCF-7): Breast cancer cell lines like MCF-7 can be studied under glucose-deprived conditions to investigate metabolic vulnerabilities and potential therapeutic targets.
2. Absence of L-Glutamine:
- Nutritional Studies with Fibroblasts: Fibroblasts, connective tissue cells, can be cultured without L-glutamine to study their ability to synthesize glutamine or understand its role in collagen production.
- Minimizing Ammonia Production in Long-Term Cultures of CHO Cells: Chinese Hamster Ovary (CHO) cells, often used in biopharmaceutical production, can benefit from the absence of L-glutamine to reduce ammonia buildup, which can be toxic during long-term cultures.
3. Absence of Phenol Red:
- Avoiding Estrogenic Effects in Prostate Cancer Cells (LNCaP): Prostate cancer cell lines like LNCaP are hormone-sensitive, and removing phenol red helps avoid estrogenic interference.
- Fluorescence Studies with Endothelial Cells (HUVECs): Human Umbilical Vein Endothelial Cells (HUVECs) used in fluorescence microscopy or flow cytometry require phenol red-free medium to avoid autofluorescence interference.
4. Absence of Sodium Bicarbonate:
- CO2-Independent Systems for Neural Stem Cells: Neural stem cells can be cultured in a controlled pH environment without needing a CO2 incubator, which is useful for certain experimental setups.
- Controlled pH Studies with Muscle Cells (C2C12): C2C12 myoblasts (muscle precursor cells) can be used in experiments where a stable pH is needed without relying on a CO2 atmosphere, allowing for precise pH control.
Overall, MBP02 MCDB 131 medium (Modification 2) is tailored for specific experimental needs where the absence of these components is crucial for the integrity and accuracy of the study, particularly in specialized cell types like HUVECs, MCF-7, LNCaP, CHO, and C2C12 cells. Results may vary based on the specific applications and requirements for studies. Please consult experts familiar with this medium.
**Disclaimer:** Results may vary depending on specific cell lines, specifications, and experimental conditions. Consult with cell culture specialists and review comprehensive literature to tailor MBP02’s use to your research needs.
