Gamborg’s B-5 Basal Salts with Vitamins

GBP05 Gamborg’s B-5 Basal Salts With Vitamins contains micronutrients, macronutrients, and vitamins. This medium provides essential nutrients required for the growth and development of plant cells, tissues, and organs in vitro.

Uses in Tissue Culture:

1. Callus Induction and Maintenance: Gamborg’s B5 medium is often used to induce and maintain callus cultures, which are undifferentiated plant cells that can be induced to form roots, shoots, or embryos under appropriate conditions.

2. Cell Suspension Cultures: It is suitable for growing plant cells in liquid suspension cultures, which are used for various purposes including secondary metabolite production and genetic transformation studies.

3. Organogenesis and Somatic Embryogenesis: The medium supports the differentiation of plant tissues into organs (roots, shoots) or somatic embryos, which can then be regenerated into whole plants.

GBP05 Gamborg’s B-5 Basal Salts With Vitamins is particularly effective for a variety of plant species. Some plants that respond well to this medium include:

1. **Legumes:** It is widely used for legumes such as soybean (Glycine max) and alfalfa (Medicago sativa) for callus induction and regeneration studies.

2. **Leafy Vegetables:** Spinach (Spinacia oleracea) and other leafy greens can be propagated using this medium.

3. **Medicinal Plants:** It is used for the tissue culture of medicinal plants like Catharanthus roseus (producing vincristine and vinblastine) and Artemisia annua (producing artemisinin).

4. **Cereals and Grains:** Sorghum (Sorghum bicolor) and maize (Zea mays) can also be cultured effectively using a B5 medium.

5. **Woody Plants:** Some woody plants, such as Populus species (poplars), are cultured using B5 medium for research in genetic transformation and propagation.